Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.
Identifieur interne : 000450 ( Main/Exploration ); précédent : 000449; suivant : 000451Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.
Auteurs : Claudia-Nicole Meisrimler [Pays-Bas, Nouvelle-Zélande] ; Alexandra J E. Pelgrom [Pays-Bas] ; Bart Oud [Pays-Bas] ; Suzan Out [Pays-Bas] ; Guido Van Den Ackerveken [Pays-Bas]Source :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2019.
Descripteurs français
- KwdFr :
- Domaines protéiques (génétique), Extinction de l'expression des gènes (immunologie), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Interactions hôte-pathogène (génétique), Laitue (génétique), Laitue (métabolisme), Maladies des plantes (microbiologie), Noyau de la cellule (métabolisme), Oomycetes (métabolisme), Oomycetes (pathogénicité), Phylogenèse (MeSH), Protéines (métabolisme), Pseudomonas (pathogénicité), Régulation de l'expression des gènes (génétique), Résistance à la maladie (MeSH), Réticulum endoplasmique (métabolisme), Stress physiologique (génétique), Transport des protéines (génétique).
- MESH :
- génétique : Domaines protéiques, Facteurs de transcription, Interactions hôte-pathogène, Laitue, Régulation de l'expression des gènes, Stress physiologique, Transport des protéines.
- immunologie : Extinction de l'expression des gènes.
- microbiologie : Maladies des plantes.
- métabolisme : Facteurs de transcription, Laitue, Noyau de la cellule, Oomycetes, Protéines, Réticulum endoplasmique.
- pathogénicité : Oomycetes, Pseudomonas.
- Phylogenèse, Résistance à la maladie.
English descriptors
- KwdEn :
- Cell Nucleus (metabolism), Disease Resistance (MeSH), Endoplasmic Reticulum (metabolism), Gene Expression Regulation (genetics), Gene Silencing (immunology), Host-Pathogen Interactions (genetics), Lettuce (genetics), Lettuce (metabolism), Oomycetes (metabolism), Oomycetes (pathogenicity), Phylogeny (MeSH), Plant Diseases (microbiology), Protein Domains (genetics), Protein Transport (genetics), Proteins (metabolism), Pseudomonas (pathogenicity), Stress, Physiological (genetics), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : Transcription Factors.
- chemical , metabolism : Proteins, Transcription Factors.
- genetics : Gene Expression Regulation, Host-Pathogen Interactions, Lettuce, Protein Domains, Protein Transport, Stress, Physiological.
- immunology : Gene Silencing.
- metabolism : Cell Nucleus, Endoplasmic Reticulum, Lettuce, Oomycetes.
- microbiology : Plant Diseases.
- pathogenicity : Oomycetes, Pseudomonas.
- Disease Resistance, Phylogeny.
Abstract
To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector-target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast-two-hybrid (Y2H) screening. Interaction of the lettuce tail-anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N-terminal NAC domain but depends on the C-terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail-anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC-domain (LsNAC069ΔNAC ). Relocalization was significantly reduced in the presence of the Ser/Cys-protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co-expression of effectors with LsNAC069ΔNAC reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses.
DOI: 10.1111/tpj.14383
PubMed: 31077456
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Diseases (microbiology)</term><term>Protein Domains (genetics)</term><term>Protein Transport (genetics)</term><term>Proteins (metabolism)</term><term>Pseudomonas (pathogenicity)</term><term>Stress, Physiological (genetics)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Domaines protéiques (génétique)</term><term>Extinction de l'expression des gènes (immunologie)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Interactions hôte-pathogène (génétique)</term><term>Laitue (génétique)</term><term>Laitue (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Noyau de la cellule (métabolisme)</term><term>Oomycetes (métabolisme)</term><term>Oomycetes (pathogénicité)</term><term>Phylogenèse (MeSH)</term><term>Protéines (métabolisme)</term><term>Pseudomonas (pathogénicité)</term><term>Régulation de l'expression des gènes (génétique)</term><term>Résistance à la maladie (MeSH)</term><term>Réticulum endoplasmique (métabolisme)</term><term>Stress physiologique (génétique)</term><term>Transport des protéines (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation</term><term>Host-Pathogen Interactions</term><term>Lettuce</term><term>Protein Domains</term><term>Protein Transport</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Domaines protéiques</term><term>Facteurs de transcription</term><term>Interactions hôte-pathogène</term><term>Laitue</term><term>Régulation de l'expression des gènes</term><term>Stress physiologique</term><term>Transport des protéines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Extinction de l'expression des gènes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Gene Silencing</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Endoplasmic Reticulum</term><term>Lettuce</term><term>Oomycetes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription</term><term>Laitue</term><term>Noyau de la cellule</term><term>Oomycetes</term><term>Protéines</term><term>Réticulum endoplasmique</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Oomycetes</term><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Oomycetes</term><term>Pseudomonas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phylogenèse</term><term>Résistance à la maladie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector-target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast-two-hybrid (Y2H) screening. Interaction of the lettuce tail-anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N-terminal NAC domain but depends on the C-terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail-anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC-domain (LsNAC069<sup>Δ</sup><sup>NAC</sup> ). Relocalization was significantly reduced in the presence of the Ser/Cys-protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co-expression of effectors with LsNAC069<sup>Δ</sup><sup>NAC</sup> reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31077456</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><Volume>99</Volume><Issue>6</Issue><PubDate><Year>2019</Year><Month>09</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.</ArticleTitle><Pagination><MedlinePgn>1098-1115</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/tpj.14383</ELocationID><Abstract><AbstractText>To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector-target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast-two-hybrid (Y2H) screening. Interaction of the lettuce tail-anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N-terminal NAC domain but depends on the C-terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail-anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC-domain (LsNAC069<sup>Δ</sup><sup>NAC</sup> ). Relocalization was significantly reduced in the presence of the Ser/Cys-protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co-expression of effectors with LsNAC069<sup>Δ</sup><sup>NAC</sup> reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses.</AbstractText><CopyrightInformation>© 2019 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meisrimler</LastName><ForeName>Claudia-Nicole</ForeName><Initials>CN</Initials><Identifier Source="ORCID">0000-0002-3571-245X</Identifier><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>University of Canterbury, Ilam, Private Bag 4800, Christchurch, 8041, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pelgrom</LastName><ForeName>Alexandra J E</ForeName><Initials>AJE</Initials><Identifier Source="ORCID">0000-0003-2064-7154</Identifier><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oud</LastName><ForeName>Bart</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Enza Zaden, Haling 1-E, Enkhuizen, 1602 DB, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Out</LastName><ForeName>Suzan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Enza Zaden, Haling 1-E, Enkhuizen, 1602 DB, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van den Ackerveken</LastName><ForeName>Guido</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0002-0183-8978</Identifier><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>Pi03192</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Organism" UI="C000637691">Pseudomonas cichorii</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004721" MajorTopicYN="N">Endoplasmic Reticulum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018545" MajorTopicYN="N">Lettuce</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009868" MajorTopicYN="N">Oomycetes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011549" MajorTopicYN="N">Pseudomonas</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Bremia lactucae
</Keyword><Keyword MajorTopicYN="Y">Lactuca sativa
</Keyword><Keyword MajorTopicYN="Y">Pseudomonas cichorii
</Keyword><Keyword MajorTopicYN="Y">NAC transcription factors</Keyword><Keyword MajorTopicYN="Y">drought stress</Keyword><Keyword MajorTopicYN="Y">oomycetes</Keyword><Keyword MajorTopicYN="Y">transcription factor relocalization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31077456</ArticleId><ArticleId IdType="doi">10.1111/tpj.14383</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Adam, Z. 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Pelgrom</name><name sortKey="Van Den Ackerveken, Guido" sort="Van Den Ackerveken, Guido" uniqKey="Van Den Ackerveken G" first="Guido" last="Van Den Ackerveken">Guido Van Den Ackerveken</name></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Meisrimler, Claudia Nicole" sort="Meisrimler, Claudia Nicole" uniqKey="Meisrimler C" first="Claudia-Nicole" last="Meisrimler">Claudia-Nicole Meisrimler</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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